CELL METABOLISM. NON-INVASIVE MEASUREMENT OF BROWN FAT METABOLISM BASED ON OPTOACOUSTIC IMAGING OF HEMOGLOBIN GRADIENTS
Josefine Reber, Monja Willershäuser, Angelos Karlas, Korbinian Paul-Yuan, Gael Diot, Tobias Fromme, Saak Ovsepian, Nicolas Beziere, Elena Dubikovskaya, Dimitrios Karampinos, Christina Holzapfel, Hans Hauner, Martin Klingenspor, Vasilis Ntziachristos. (23-02-2018).Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation, 2018
Research Area A
Metabolism is a fundamental process of life. However, non-invasive measurement of local tissue metabolism is limited today by a deficiency in adequate tools for in vivo observations. We designed a multi-modular platform that explored the relation between local tissue oxygen consumption, determined by label-free optoacoustic measurements of hemoglobin, and concurrent indirect calorimetry obtained during metabolic activationc of brown adipose tissue (BAT). By studying mice and humans, we show how video-rate handheld multi-spectral optoacoustic tomography (MSOT) in the 700-970 nm spectral range enables non-invasive imaging of BAT activation, consistent with Positron Emission Tomography findings. Moreover, we observe BAT composition differences between healthy and diabetic tissues. The study consolidates hemoglobin as a principal label-free biomarker for longitudinal non-invasive imaging of BAT morphology and bioenergetics in situ. We also resolve water and fat components in volunteers, and contrast MSOT readouts to Magnetic Resonance Imaging data.