A CONTINUOUSLY INFUSED MICROFLUIDIC RADIOASSAY SYSTEM FOR THE CHARACTERIZATION OF CELLULAR PHARMACOKINETICS
Zhen Liu, Ziying Jian, Qian Wang, Tao Cheng, Benedikt Feuerecker, Markus Schwaiger, Sung-Cheng Huang, Sibylle I. Ziegler, Kuangyu Shi. (12-04-2016).Dept. Nuclear Medicine, Technische Universität München, Munich, Germany Dept. Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, USA
Research Area Z
Measurement of cellular tracer uptake is widely applied to understand the physiological status of cells and their interactions with imaging agents and pharmaceuticals. In-culture measurements have the advantage of less stress to cells. However, the tracer solution still needs to be loaded, unloaded and purged from the cell culture during the measurements. Here, we propose a continuously infused microfluidic radioassay (CIMR) system for continuous in-culture measurement of cellular uptake. The system was tested to investigate the influence of glucose concentration in cell culture medium on the 2-deoxy-2-(18F)fluoro-D-glucose (18F-FDG) uptake kinetics.