FMT-PCCT: HYBRID FLUORESCENCE MOLECULAR TOMOGRAPHY—X-RAY PHASE-CONTRAST CT IMAGING OF MOUSE MODELS
Pouyan Mohajerani, Alexander Hipp, Marian Willner, Mathias Marschner, Marija Trajkovic-Arsic, Xiaopeng Ma, Neal C. Burton, Uwe Klemm, Karin Radrich, Vladimir Ermolayev, Stratis Tzoumas, Jens T. Siveke, Martin Bech, Franz Pfeiffer, Vasilis Ntziachristos. (24-03-2014).IEEE TRANSACTIONS ON MEDICAL IMAGING, VOL. 33, NO. 7, JULY 2014, 2014, DOI 10.1109/TMI.2014.2313405
Research Area A
The implementation of hybrid ﬂuorescence molecular tomography (FMT) and X-ray computed tomography (CT) has been shown to be a necessary development, not only for combining anatomical with functional and molecular contrast, but also for generating optical images of high accuracy. FMT affords highly sensitive 3-D imaging of ﬂuorescence bio-distribution, but in standalone form it offers images of low resolution. It was shown that FMT accuracy signiﬁcantly improves by considering anatomical priors from CT. Conversely, CT generally suffers from low soft tissue contrast. Therefore utilization of CT data as prior information in FMT inversion is challenging when different internal organs are not clearly differentiated. Instead, we combined herein FMT with emerging X-ray phase-contrast CT (PCCT). PCCT relies on phase shift differences in tissue to achieve soft tissue contrast superior to conventional CT. We demonstrate for the ﬁrst time FMT-PCCT imaging of different animal models, where FMT and PCCT scans were performed in vivo and ex vivo, respectively.
The results show that FMT-PCCT expands the potential of FMT in imaging lesions with otherwise low or no CT contrast, while retaining the cost beneﬁts of CT and simplicity of hybrid device realizations. The results point to the most accurate FMT performance to date.